每2
昼夜节律
医学
促炎细胞因子
甲状腺球蛋白
时钟
自身免疫性甲状腺炎
甲状腺
甲状腺炎
内科学
内分泌学
生物钟
免疫学
炎症
作者
Jinrong Fu,Zihao Fan,Liang He,Qian Liu,He Liu,Yushu Li,Haixia Guan
出处
期刊:European thyroid journal
[S. Karger AG]
日期:2023-08-07
卷期号:12 (5)
摘要
Objective A vicious cycle between circadian disruption and escalating immune responses has been described in diverse inflammatory disease. The current study aimed to explore the role of circadian clock disruption in autoimmune thyroiditis (AIT). Methods Thirty AIT patients and 30 controls were enrolled and biopsied for thyroid tissues. Alterations of core clock genes expression in AIT thyroid tissues, and its association with serum and tissue inflammatory biomarkers were assessed. For animal studies, C57BL/6J mice administered with porcine thyroglobulin or PBS (as control) combined with adjuvants were sacrificed at four time points to investigate the circadian characteristic of experimental autoimmune thyroiditis (EAT). Light shift (LS) conditions were used to explore the influence of external circadian disturbance on EAT. Results The expression of clock genes BMAL1 and PER2 was significantly reduced in thyroid tissues from AIT patients and was negatively correlated to levels of thyroid peroxidase antibodies. In mouse models, diurnal fluctuations of proinflammatory cytokines were demonstrated, and further exposing mice to LS led to overproduction of TNF-α, IFN-γ, and anti-thyroglobulin antibodies. Circadian analysis revealed significant oscillations of Bmal1 , Clock , Per2 , Cry1 , Ror, and Rev-erb , which was broadly disturbed in EAT, LS, and EAT + LS groups. Conclusions This study demonstrates that expression pattern of clock genes was disrupted in AIT thyroid, and chronic circadian disruption may aggravate the inflammatory responses in AIT. Whether maintaining a regular circadian rhythm can alleviate autoimmune thyroid diseases warrants further research.
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