屎肠球菌
细菌素
生物
益生菌
微生物学
肠球菌
单核细胞增生李斯特菌
乳酸乳球菌
樱乳杆菌
大肠杆菌
乳酸菌
细菌
抗菌剂
基因
遗传学
乳酸
抗生素
作者
Charles M. A. P. Franz,Bruno Pot,María Guadalupe Vizoso-Pinto,Achille Arini,Raffaella Coppolecchia,Wilhelm H. Holzapfel
出处
期刊:Beneficial Microbes
[Wageningen Academic Publishers]
日期:2017-01-01
卷期号:15 (2): 211-225
标识
DOI:10.1163/18762891-bja00005
摘要
Abstract Enterococcus faecium SF68 (SF68) is a well-known probiotic with a long history of safe use. Recent changes in the taxonomy of enterococci have shown that a novel species, Enterococcus lactis , is closely related with E. faecium and occurs together with other enterococci in a phylogenetically well-defined E. faecium species group. The close phylogenetic relationship between the species E. faecium and E. lactis prompted a closer investigation into the taxonomic status of E. faecium SF68. Using phylogenomics and ANI, the taxonomic analysis in this study showed that probiotic E. faecium SF68, when compared to other E. faecium and E. lactis type and reference strains, could be re-classified as belonging to the species E. lactis . Further investigations into the functional properties of SF68 showed that it is potentially capable of bacteriocin production, as a bacteriocin gene cluster encoding the leaderless bacteriocin EntK1 together with putative Lactococcus lactis bacteriocins LsbA, and LsbB-like putative immunity peptide (LmrB) were found located in an operon on plasmid pF9. However, bacteriocin expression was not studied. Competitive exclusion experiments in co-culture over 7 days at 37 °C showed that the probiotic SF68 could inhibit the growth of specific E. faecium and Listeria monocytogenes strains, while showing little or no inhibitory activity towards an entero-invasive Escherichia coli and a Salmonella Typhimurium strain, respectively. In cell culture experiments with colon carcinoma HT29 cells, the probiotic SF68 was also able to strain-specifically inhibit adhesion and/or invasion of enterococcal and L. monocytogenes strains, while such adhesion and invasion inhibition effects were less pronounced for E. coli and Salmonella strains. This study therefore provides novel data on the taxonomy and functional properties of SF68, which can be reclassified as Enterococcus lactis SF68, thereby enhancing the understanding of its probiotic nature.
科研通智能强力驱动
Strongly Powered by AbleSci AI