Lipase Production from Aspergillus niger Used as a Biocatalyst for Transformation of Used Cooking Oil into Biodiesel

Aspergillus niger lipase was employed in transesterification of used cooking oil into biodiesel.Fungus cultivated in molasses mineral medium with tryptone as the nitrogen source for 5 days at 37 °C and 6.0 pH, strain produced a maximum lipase concentration of 29.39 (U/mL).Effect of metal ions, pH, pH stability, temperature, and thermostability on lipase activity were examined.Transformation of used oil into biodiesel was carried out by employing lipase produced in the current work.Maximal fatty acid methyl ester formation was noted after 18 h, 45 °C, and a 1:05 oil-to-methanol ratio.Agitation speed and enzyme concentration also enhanced biodiesel yield.Biodiesel production reached 92% under optimal conditions.Biodiesel production is simplified by using on-site produced lipase as catalyst.These findings could be significant advancement in biodiesel production from its commercialization prospects.Novelty Statement | Utilization of onsite produced lipase from locally isolated strain to produce biodiesel from waste cooking oil.