纳米孔测序
计算生物学
生物
限制
抄写(语言学)
DNA测序
基因组
转录组
基因
遗传学
基因表达
工程类
机械工程
语言学
哲学
作者
Leena Putzeys,Danish Intizar,Rob Lavigne,Maarten Boon
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 207-235
标识
DOI:10.1007/978-1-0716-3798-2_14
摘要
Detailed transcription maps of bacteriophages are not usually explored, limiting our understanding of molecular phage biology and restricting their exploitation and engineering. The ONT-cappable-seq method described here brings phage transcriptomics to the accessible nanopore sequencing platform and provides an affordable and more detailed overview of transcriptional features compared to traditional RNA-seq experiments. With ONT-cappable-seq, primary transcripts are specifically capped, enriched, and prepared for long-read sequencing on the nanopore sequencing platform. This enables end-to-end sequencing of unprocessed transcripts covering both phage and host genome, thus providing insight on their operons. The subsequent analysis pipeline makes it possible to rapidly identify the most important transcriptional features such as transcription start and stop sites. The obtained data can thus provide a comprehensive overview of the transcription by your phage of interest.
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