An Overview of Monolithic Column: Types, Parameters and Applications

The column is the main component for chromatographic separation. Nowadays, monolithic columns are graining more popularity in the field of separation media for liquid chromatography. The monolith columns possess great potential as compared to the conventional packed column in terms of preparing complex mixtures. These columns provide various properties like higher permeability, high-efficiency fast separations, high flow rate with lower backpressure, fast mass transfer kinetics with a high binding capacity. It is categories into three columns and they are organic monolithic column, inorganic monolithic column and hybrid monolithic column and all three types of monolithic column differ through their porous properties. In this review, the various advantage of the high-efficiency monolithic column with recent advances, the origin of the concept, the various parameter of the monolithic stationary phase and the application of monolithic columns are illustrated. It is better column in comparison of selectivity, reproducibility and performance. Keywords: Monolithic column, Packed columns, Inorganic and organic monolithic column, Column parameters, Pharmaceutical Applications