Romboutsia Lituseburensis Jcm1404 Supplementation Modulates Gut Microbiota Composition And Influences Endothelial Function In Rats

Many studies have indicated the relative abundance of Romboutsia was hgher in obese individuals, while some evidence showed it was reduced with exercise intervention. A correlation between Romboutsia and endothelial function was found in our previous study. However, the mechanism by which exercise-mediated changes in gut microbiota on endothelial function remains unclear. PURPOSE: To demonstrate whether strain Romboutsia lituseburensis JCM1404 (RL-JCM1404) supplementation modulates gut microbiota composition and endothelial function in healthy or obesity rats. METHODS: There were four groups, named SD (standard diet) control group, SD RL-JCM1404 group, HF (High-fat diet) control group, HF RL-JCM1404 group. All rats treated with antibiotic for 2 ml per day for 5 consecutive days prior to transplantation. The treatment groups were orally administered 109cfu/ml every two days for 6 times. 16S rRNA sequencing was used to determine changes of cecal microbiome. Endothelium-dependent vasodilation was induced by acetylcholine (0.01-10 mmol/L) in mesenteric arteries. RESULTS: RL-JCM1404 could modulate the gut microbiota, a decreased Romboutsia, Clostridium_sensu_stricto_1, an increased Colidextribacter, Papilliibacter, Intestinimonas at genus level compared with the SD control group. While a decreased Romboutsia, Lactobacillus, Turicibacter, Staphylococcus, Clostridium_sensu_stricto_1, GCA-900066575, an increased Bilophila compared with the HF control group. Tax4Fun predicted metabolism of cofactors and vitamins, cardiovascular diseases were enriched at the second level in the SD RL-JCM1404 group compared with the SD control group. While carbohydrate metabolism, replication and repair, endocrine and metabolic diseases, nervous system were enriched in the HF control group compared with the HF RL-JCM1404 group. The second-order mesenteric arteries was impaired in the SD RL-JCM1404 group (67.5 ± 4.79 VS. 51.4 ± 4.37, p < 0.05) compared with SD control group in the maximum response to acetylcholine, but there were no statistical differences between HF control and HF RL-JCM1404 groups. CONCLUSIONS: RL-JCM1404 supplementation could affect endothelial function via modulating gut microbiota composition and metabolism in standard diet rats.