EXTRACORPOREAL CARDIOPULMONARY RESUSCITATION WITH THERAPEUTIC HYPOTHERMIA MITIGATES KIDNEY INJURY AFTER CARDIAC ARREST IN RATS

医学 体温过低 心肺复苏术 体外心肺复苏 复苏 麻醉 体外循环 坏死性下垂 氧化应激 免疫学 细胞凋亡 程序性细胞死亡 内科学 生物 生物化学
作者
Shuhong Chen,Jie Yu,Peng-Hui Xue,Feilong Hei,Yulong Guan
出处
期刊:Shock [Ovid Technologies (Wolters Kluwer)]
卷期号:60 (2): 315-324 被引量:1
标识
DOI:10.1097/shk.0000000000002171
摘要

Many patients with cardiac arrest (CA) experience severe kidney injury after the return of spontaneous circulation. This study aimed to compare the renal protective effect of conventional cardiopulmonary resuscitation (CCPR), extracorporeal cardiopulmonary resuscitation (ECPR), and ECPR with therapeutic hypothermia (ECPR+T) in a CA rat model. Twenty-four adult male Sprague-Dawley rats were randomly and equally allocated into the sham, CCPR, ECPR, and ECPR+T groups. The sham group underwent basic surgical procedures without asphyxia-induced CA. The other three groups were treated with asphyxiation to establish the CA model. Subsequently, they were rescued using three different therapeutic methods. The end points were 1 h after return of spontaneous circulation or death. Renal injury was evaluated by histopathology. Oxidative stress, endoplasmic reticulum stress, necroptosis, inflammatory, and apoptosis-related genes, and proteins were detected using western blotting, ELISA, and assay kit. Compared with CCPR, ECPR and ECPR+T alleviated oxidative stress by upregulating nuclear factor erythroid 2-related factor 2, superoxide dismutase, glutathione and downregulating heme oxygenase-1, and malondialdehyde. Expression of endoplasmic reticulum stress-related proteins, glucose-regulated protein 78, and CCAAT/enhancer-binding protein homologous protein was lower in ECPR and ECPR+T groups than that in the CCPR group, along with levels of TNF-α, IL-6, and IL-β, and necroptosis proteins (receptor-interacting serine/threonine kinases 1 and 3). Furthermore, the ECPR and ECPR+T groups had significantly increased B-cell lymphoma 2 and decreased B-cell lymphoma 2-associated X levels compared with the CCPR group. Extracorporeal cardiopulmonary resuscitation and ECPR+T alleviate kidney damage after CA in rats compared with CCPR. Furthermore, ECPR+T had a better renal protective effect.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
机智的紫丝完成签到,获得积分10
1秒前
李爱国应助归仔采纳,获得10
2秒前
在我梦里绕完成签到,获得积分10
2秒前
科研通AI2S应助碎冰冰采纳,获得10
2秒前
科研通AI2S应助碎冰冰采纳,获得10
2秒前
郑先生完成签到 ,获得积分20
3秒前
4秒前
南楼小阁主完成签到,获得积分10
5秒前
8秒前
归仔完成签到,获得积分10
8秒前
xvzhenyuan发布了新的文献求助10
10秒前
酷酷学发布了新的文献求助10
11秒前
loulan完成签到,获得积分10
12秒前
星忆眠完成签到,获得积分10
12秒前
Orange应助樊书南采纳,获得10
15秒前
wy.he举报我不可爱jx求助涉嫌违规
15秒前
ximomm完成签到,获得积分10
17秒前
顺心的梨愁完成签到 ,获得积分10
17秒前
小景完成签到,获得积分20
18秒前
chen完成签到,获得积分10
19秒前
20秒前
飘逸问薇完成签到 ,获得积分10
21秒前
21秒前
TX_W发布了新的文献求助10
22秒前
小小康康完成签到,获得积分10
22秒前
piaoyingzhiyu发布了新的文献求助30
26秒前
Lazarus_x发布了新的文献求助10
26秒前
yema完成签到 ,获得积分10
28秒前
30秒前
31秒前
浅是宝贝完成签到 ,获得积分10
31秒前
dengqin完成签到 ,获得积分10
32秒前
JamesPei应助Lazarus_x采纳,获得10
35秒前
哭泣剑封完成签到,获得积分10
37秒前
37秒前
38秒前
小学生的练习簿完成签到,获得积分10
39秒前
41秒前
xuxu发布了新的文献求助10
41秒前
科研通AI2S应助北辰采纳,获得10
41秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Genera Insectorum: Mantodea, Fam. Mantidæ, Subfam. Hymenopodinæ (Classic Reprint) 800
Ethnicities: Media, Health, and Coping 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3085924
求助须知:如何正确求助?哪些是违规求助? 2738890
关于积分的说明 7552090
捐赠科研通 2388595
什么是DOI,文献DOI怎么找? 1266658
科研通“疑难数据库(出版商)”最低求助积分说明 613539
版权声明 598591