透明质酸
生物相容性
化学
细胞毒性
席夫碱
体内
控制释放
核化学
药品
抗真菌药
药理学
色谱法
体外
生物化学
有机化学
高分子化学
微生物学
抗真菌
医学
生物
生物技术
解剖
作者
Wenqian Wang,Depeng Shi,Yongfei Zhang,Wenhui Li,Feng Li,Feng Huang,Lichun Ma,Chao Yang,Zhi Peng,Guojun Song,Hongbo Zeng,Lixin Xie
标识
DOI:10.1016/j.ijbiomac.2023.125341
摘要
Drug-loaded injectable hydrogels have been studied widely in biomedical technology while the stable long-term controlled drug release and cytotoxicity are challenges. In this work, an injectable hydrogel with good swelling resistance was in situ synthetized using aminated hyaluronic acid (NHA) and aldehyde β-cyclodextrin (ACD) via Schiff base reaction. The composition, morphology and mechanical property were characterized with FTIR, 13C NMR, SEM and rheology test, respectively. Voriconazole (VCZ) and Endophthalmitis was selected as a model drug and disease, respectively. The drug release, cytotoxicity and antifungal properties were detected in vitro. The results showed a long-term (> 60 days) drug release was realized, the NHA/ACD2/VCZ presented a zero-order release in the later stage. The cytotoxicity of NHA/ACD was detected by live/dead staining assay and Cell Counting Kit-8 (CCK-8). The survival rate of adult retina pigment epithelial cell line-19 (ARPE-19) was over 100 % after 3 d, it indicated a good cytocompatibility. The antifungal experiment presented samples had antifungal property. Biocompatibility in vivo proved NHA/ACD2 had no adverse effects on ocular tissues. Consequently, the injectable hydrogel based on hyaluronic acid prepared by Schiff base reaction provides a new option for long-term controlled drug release in the course of disease treatment from a material perspective.
科研通智能强力驱动
Strongly Powered by AbleSci AI