淘选
噬菌体展示
肽库
肽
癌症
分子生物学
生物
幽门螺杆菌
胃粘膜
免疫组织化学
肽序列
胃
生物化学
免疫学
基因
遗传学
作者
Wen Jie Zhang,Yan Xia Sui,Arun Budha,Jian Zheng,Xuejun Sun,Ying Chun Hou,Thomas D. Wang,Shao Ying Lu
标识
DOI:10.3748/wjg.v18.i17.2053
摘要
To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric cancer.A peptide screen was performed by biopanning the PhD-12 phage display library, clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer tissues. Tumor-targeted binding of selected peptides was confirmed by bound phage counts, enzyme-linked immunosorbent assay, competitive inhibition, fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer tissues.Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal- appearing gastric mucosa. After the third round of positive screening, the peptide sequence AADNAKTKSFPV (AAD) appeared in 25% (12/48) of the analyzed phages. For the control peptide, these values were 6.8 ± 2.3, 5.1 ± 1.7, 3.5 ± 2.1, 4.6 ± 1.9 and 1.1 ± 0.5, respectively. The values for AAD peptide were statistically significant (P < 0.01) for gastric cancer as compared with other histological classifications and control peptide.A novel peptide is discovered to have a specific binding activity to gastric cancer, and can be used to distinguish neoplastic from normal gastric mucosa, demonstrating the potential for early cancer detection on endoscopy.
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