环介导等温扩增
量油尺
荧光染料
分子生物学
重组酶聚合酶扩增
生物
假阳性悖论
聚合酶链反应
DNA
色谱法
病毒学
化学
生物化学
计算机科学
基因
机器学习
尿
标识
DOI:10.1016/j.diagmicrobio.2010.08.026
摘要
A combined loop-mediated isothermal amplification lateral flow dipstick (LAMP-LFD) format was evaluated in the detection of human infective trypanosome DNA from clinical samples. The LAMP-LFD showed analytical sensitivity equivalent to 0.01 tryps/mL, levels that were identical to using gel electrophoresis and SYBR Green I dye. The LAMP-LFD showed superior specificity to SYBR Green I when supernatant prepared from boiled human biological samples was used as template. These results indicate that the use of nonspecific DNA intercalators may produce false positives when partially processed templates are used. The LAMP-LFD format presented here is simple, rapid, and has future potential use in diagnosis of sleeping sickness.
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