拟南芥
液泡
拟南芥
细胞生物学
生物
细胞器
绿色荧光蛋白
液泡蛋白分选
蛋白质亚细胞定位预测
细胞质
生物化学
基因
突变体
作者
Paul R. Hunter,Christian Craddock,Sara Di Benedetto,Lynne M. Roberts,Lorenzo Frigerio
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2007-09-28
卷期号:145 (4): 1371-1382
被引量:196
标识
DOI:10.1104/pp.107.103945
摘要
Abstract We generated fusions between three Arabidopsis (Arabidopsis thaliana) tonoplast intrinsic proteins (TIPs; α-, γ-, and δ-TIP) and yellow fluorescent protein (YFP). We also produced soluble reporters consisting of the monomeric red fluorescent protein (RFP) and either the C-terminal vacuolar sorting signal of phaseolin or the sequence-specific sorting signal of proricin. In transgenic Arabidopsis leaves, mature roots, and root tips, all TIP fusions localized to the tonoplast of the central vacuole and both of the lumenal RFP reporters were found within TIP-delimited vacuoles. In embryos from developing, mature, and germinating seeds, all three TIPs localized to the tonoplast of protein storage vacuoles. To determine the temporal TIP expression patterns and to rule out mistargeting due to overexpression, we generated plants expressing YFP fused to the complete genomic sequences of the three TIP isoforms. In transgenic Arabidopsis, γ-TIP expression was limited to vegetative tissues, but specifically excluded from root tips, whereas α-TIP was exclusively expressed during seed maturation. δ-TIP was expressed in vegetative tissues, but not root tips, at a later stage than γ-TIP. Our findings indicate that, in the Arabidopsis tissues analyzed, two different vacuolar sorting signals target soluble proteins to a single vacuolar location. Moreover, TIP isoform distribution is tissue and development specific, rather than organelle specific.
科研通智能强力驱动
Strongly Powered by AbleSci AI