MICRO-DETERMINATION OF CARBONIC ANHYDRASE ACTIVITY IN ANIMAL TISSUE1

A modified manometric micro-method for the determination of carbonic anhydrase activity, using a pure carbonic anhydrase preparation for the definition of unit enzyme activity, was examined and established as a sensitive, accurate and practical method suitable for biological studies. Estimations of the carbonic anhydrase activity in the uteri of some mammalian species indicate that this method is useful for further investigations of this enzyme.