已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Enhancement of angiogenesis, tumor growth, and metastasis by transfection of vascular endothelial growth factor into LoVo human colon cancer cell line.

转移 转染 血管生成 血管内皮生长因子 生物 细胞培养 癌症研究 克隆(Java方法) 癌细胞 细胞生长 病理 血管内皮生长因子A 分子生物学 癌症 医学 基因 血管内皮生长因子受体 生物化学 遗传学
作者
Yoko Kondo,Shigeki Arii,Akira Mori,Masaharu Furutani,Tsutomu Chiba,Masayuki Imamura
出处
期刊:PubMed 卷期号:6 (2): 622-30 被引量:100
链接
标识
摘要

The expression of vascular endothelial growth factor (VEGF), a highly potent angiogenic molecule, is thought to be correlated with the development of colon cancer; however, direct evidence for a role of VEGF in metastasis is lacking. This study was designed to more directly establish the role of VEGF in the growth and metastasis of human colon cancer using a genetically engineered cancer cell line. A stable VEGF gene-transfected human colon cancer cell line, LoVo, was made by genetic manipulation using eukaryotic expression constructs designed to express the complete VEGF121 cDNA in the sense orientation. Transfected clones were screened for VEGF121 mRNA expression by Northern blot analysis and for VEGF121 protein expression by Western blot analysis. Consequently, we obtained S17 cells that expressed a high level of both VEGF mRNA and VEGF protein. A vector-transfected clone (V7 cell) was used as a control. The experiment with the dorsal air sac method revealed that S17 cells elicited a stronger directional out-growth of capillaries than V7 cells. S17 cells formed faster-growing tumors than did V7 cells when xenografted s.c. into nude mice, although there was no significant difference in their in vitro proliferation. Tumors derived from S17 cells had more vascularity, as assessed by counting capillary vessels after staining with factor VIII, than did tumors derived from V7 cells (P < 0.05). With regard to the metastatic potential, S17 cells exhibited a higher capacity for both hepatic metastasis after the splenic portal inoculation and peritoneal dissemination after i.p. injection than did V7 cells. However, S17 cells showed no apparent metastasis, despite their rapid growth after orthotopic implantation. In conclusion, the present study showed clearly that VEGF plays an important role in cancer growth due to stimulation of angiogenesis by accelerating cell growth after reaching the target organs.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Doki发布了新的文献求助10
刚刚
风采发布了新的文献求助10
1秒前
荒废完成签到,获得积分10
4秒前
dllneu完成签到,获得积分10
6秒前
6秒前
yls完成签到,获得积分10
9秒前
木可发布了新的文献求助10
10秒前
13秒前
gxzsdf完成签到 ,获得积分10
14秒前
柚子完成签到 ,获得积分10
17秒前
17秒前
luwenxuan发布了新的文献求助10
20秒前
22秒前
Hello应助哈哈哈哈采纳,获得10
25秒前
光亮静槐完成签到 ,获得积分10
26秒前
27秒前
朴实的热狗关注了科研通微信公众号
28秒前
31秒前
小凉完成签到 ,获得积分10
32秒前
33秒前
34秒前
Kirito完成签到,获得积分0
34秒前
zero1122发布了新的文献求助10
35秒前
哈哈哈哈完成签到,获得积分10
35秒前
wangch198201完成签到 ,获得积分10
36秒前
lyy66964193完成签到,获得积分10
37秒前
哈哈哈哈发布了新的文献求助10
39秒前
清爽源智发布了新的文献求助10
39秒前
nature发布了新的文献求助10
40秒前
善学以致用应助luwenxuan采纳,获得10
40秒前
似水流年完成签到 ,获得积分10
42秒前
Doki发布了新的文献求助10
43秒前
lutiantian发布了新的文献求助10
45秒前
酷波er应助瑾钰满糖采纳,获得10
46秒前
47秒前
卡皮巴拉完成签到,获得积分10
47秒前
清爽源智完成签到,获得积分10
50秒前
52秒前
漂亮采波完成签到,获得积分10
53秒前
YamDaamCaa应助科研顺利啦采纳,获得50
54秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976600
求助须知:如何正确求助?哪些是违规求助? 3520674
关于积分的说明 11204422
捐赠科研通 3257298
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877842
科研通“疑难数据库(出版商)”最低求助积分说明 806595