下调和上调
精液
脂质过氧化
男科
DNA甲基化
甲基化
运动性
细胞凋亡
精子
生物
化学
细胞生物学
DNA
生物化学
内分泌学
氧化应激
基因表达
医学
基因
作者
İbrahim Halil Güngör,Ahmet Tektemur,Gözde Arkalı,Serap Dayan Cinkara,Tutku Can Acısu,Recep Hakkı Koca,Ebru Önalan Etem,Şeyma Özer Kaya,Meltem Kızıl,Mustafa Sönmez,Seyfettin Gür,Zafer Çambay,Abdurrauf Yüce,Gaffari Türk
摘要
This study was carried out to investigate the effect of the semen freeze–thawing process on the functionality and molecular structure of ram spermatozoa. The temperature of pooled and diluted semen at 38°C (group 1, control) was lowered to 5°C (group 2), and it was subjected to glycerolisation–equilibration (group 3), frozen and thawed (group 4). Compared to the control, deterioration in spermatological parameters and significant increases in lipid peroxidation and global DNA methylation levels were observed in groups 3 and 4. When compared with the control, significant downregulation in the levels of miR-485 of group 2, miR-29a of group 3 and let-7a, miR-485 and miR-29a of group 4, and significant upregulation in the levels of miR-107 of group 3 and miR-127 of groups 3 and 4 were detected. In comparison to the control, significant upregulation in the levels of CatSper1, CatSper2, CatSper3, CatSper4, ANO1 and TRPM3 of group 2, CatSper4, ANO1 and TRPM3 of group 3 and KCNJ11 of group 4, and significant downregulation in the CatSper 3 level of group 4 were determined. As a result, the semen freeze–thawing process causes motility and morphological disorders in rams. This may be due to molecular changes associated with lipid peroxidation in spermatozoa.
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