石墨烯
适体
量子点
DNA
荧光
线性范围
信号(编程语言)
化学
检出限
材料科学
纳米技术
计算机科学
分子生物学
物理
色谱法
生物
生物化学
光学
程序设计语言
作者
Qi Wang,Fangyuan Zhao,Qingli Yang,Wei Wu
标识
DOI:10.1016/j.snb.2021.130387
摘要
Aflatoxin B1 (AFB1) is the most toxic metabolite of mycotoxins and constantly contaminates food crops, which seriously endangers human health. Therefore, it is essential to explore a rapid and sensitive method for AFB1 detection. Herein, a fluorescence aptasensor based on DNA walker, DNA tetrahedron nanostructures (DTNs) and network hybridization chain reaction (HCR) for monitoring AFB1 was proposed. DNA walker structure was employed as signal amplifier induced by targets AFB1 where DNA walker chain could walk on a three-dimensional track of gold nanoparticles (AuNPs) and combine complementary sequences of DTNs, triggering the cutting of endonuclease and releasing a lot of DTNs. DTNs were fabricated by four single-stranded DNA, which have multiple fulcrums. DTNs provided multiple reaction orientations for HCR and improved the assembly rate of graphene oxide quantum dots (GQDs) based nanotree. In presence of target AFB1, target AFB1 would bind with aptamers and trigger secondary signal amplification to achieve sensitive detection. This aptasensor could sensitively detect AFB1 in a linear range of 1−1000 pg/mL with a detection limit as low as 0.492 pg/mL. In addition, this method can also be employed in monitoring other hazardous substances.
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