亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Network pharmacology integrated with molecular docking reveals the common experiment-validated antipyretic mechanism of bitter-cold herbs

解热药 药理学 传统医学 人参 对接(动物) 中医药 化学 草本植物 嗜中性 医学 草药 病理 护理部 替代医学 止痛药
作者
Weiwei Zhou,Yifei Dai,Jing Meng,Pengqian Wang,Yin Wu,Dai Li,Miao Zhang,Xinggang Yang,Shujun Xu,Shibin Feng,Hai-Ru Huo
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:274: 114042-114042 被引量:12
标识
DOI:10.1016/j.jep.2021.114042
摘要

Bitter-cold herbs have been used to clearing heat and expelling damp in clinical practice in China for thousands of years. Aim of the study: This study aimed to investigate the common molecular mechanism of bitter-cold herbs through network pharmacology analysis, molecular docking and experimental validation in vivo. Network pharmacological analysis integrated with molecular docking was employed to identify the active compounds and core action targets of the bitter-cold herbs. Then, the yeast-induced pathological model was established, and the antipyretic effect of the herbs was evaluated by checking rectal temperatures of the mice hourly. Lastly, the protein expression of core targets was examined to reveal the antipyretic mechanism. A total of 52 lead compounds from the four bitter-cold herbs, Phellodendri Chinensis Cortex (PCC), Sophorae Flavescentis Radix (SFR), Gentianae Radix Et Rhozima (GRER) and Coptidis Rhizoma (CR), and 248 compounds-related targets were screened out with PTGS2 ranking the first. The results from molecular docking showed that 22 compounds adopted the same orientation as aspirin and had an excellent stability in the active site pocket of PTGS2. Furthermore, these herbs exerted potential therapeutic effects through 38 related pathways. On the other hand, the outcome of animal experiments showed that they could significantly attenuate the yeast-induced mice fever with dose-dependent relationship. Further experimental results demonstrated that administration of yeast suspension raised protein expression of PTGS2 significantly, which was evidently inhibited in the high or low-dose groups of GRER as well as in the low-dose group of SFR (P < 0.01) though a higher expression of PTGS2 was shown in the low-dose group of CR compared with FM group (P < 0.01). The bitter-cold herbs can alleviate fever response and their antipyretic effect may mainly be attributed to regulating the expression of PTGS2 after the formation of ligand-receptor/PTGS2 complexes, and their active compounds might be nominated as antipyretic lead-ligand candidates.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
充电宝应助chiyudoubao采纳,获得10
6秒前
Lucas应助gulmira采纳,获得10
25秒前
wanci应助Dr.Leon采纳,获得10
34秒前
34秒前
单纯的雅香完成签到,获得积分10
40秒前
44秒前
wangyang完成签到,获得积分10
50秒前
南宫雪完成签到 ,获得积分10
53秒前
无私航空发布了新的文献求助50
58秒前
lixuebin完成签到 ,获得积分10
1分钟前
wangyang发布了新的文献求助10
1分钟前
1分钟前
无私航空完成签到,获得积分10
1分钟前
1分钟前
gulmira发布了新的文献求助10
1分钟前
1分钟前
1分钟前
可爱的你发布了新的文献求助60
1分钟前
WW应助gulmira采纳,获得10
1分钟前
1分钟前
2分钟前
jason完成签到,获得积分10
2分钟前
2分钟前
jason发布了新的文献求助10
2分钟前
chiyudoubao发布了新的文献求助10
2分钟前
可爱的你完成签到,获得积分10
2分钟前
2分钟前
chiyudoubao完成签到,获得积分10
2分钟前
小二郎应助桃园奈奈露采纳,获得10
2分钟前
2分钟前
Hans完成签到,获得积分10
2分钟前
Glngar关注了科研通微信公众号
2分钟前
3分钟前
3分钟前
诚心的信封完成签到 ,获得积分10
3分钟前
decade发布了新的文献求助10
3分钟前
3分钟前
sunny完成签到,获得积分10
3分钟前
隐形曼青应助thousandlong采纳,获得10
3分钟前
3分钟前
高分求助中
Sustainability in Tides Chemistry 1500
Handbook of the Mammals of the World – Volume 3: Primates 805
拟南芥模式识别受体参与调控抗病蛋白介导的ETI免疫反应的机制研究 550
Gerard de Lairesse : an artist between stage and studio 500
Digging and Dealing in Eighteenth-Century Rome 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Manual of Sewer Condition Classification 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3068019
求助须知:如何正确求助?哪些是违规求助? 2722010
关于积分的说明 7475939
捐赠科研通 2369097
什么是DOI,文献DOI怎么找? 1256116
科研通“疑难数据库(出版商)”最低求助积分说明 609454
版权声明 596795