葡萄糖氧化酶
化学
米氏-门汀动力学
酶动力学
催化作用
组合化学
生物化学
酶
活动站点
酶分析
作者
Juan Han,Wenrui Huang,Man Zhao,Jiacong Wu,Yuanyuan Li,Yanli Mao,Lei Wang,Yun Wang
标识
DOI:10.1016/j.colsurfb.2021.112034
摘要
Herein, a glucose [email protected] composite (3-MPBA/[email protected]) with enhanced enrichment was enabled the rapid encapsulation of glucose oxidase (GOx) into microporous zeolitic imidazolate framework-8 (ZIF-8) for the first time. The 3-MPBA/[email protected] not only has improved affinity and catalytic efficiency to the substrate but also can shorten the formation time. The optimum loading amount of GOx on ZIF-8 was determined to be 470 mg/g. The as-prepared 3-MPBA/[email protected] composite maintained the native conformation of the enzyme and showed excellent bioactivity, even in chemical agents or at high temperature. Furthermore, the 3-MPBA/[email protected] showed satisfactory reusability, preserving almost 80.8 % activity after 7 cycles. The Michaelis constant Km and specificity constant kcat/Km of the 3-MPBA/[email protected] were 0.03 ± 0.02 mM and 63.87 ± 1.96 s−1 mM−1, respectively, which were superior to corresponding values of free GOx. Therefore, the 3-MPBA/[email protected] displayed high catalytic efficiency, high loading efficiency and enhanced stability. Moreover, a new type of visual colorimetric sensor for screening of the diabetes was realized through the 3-MPBA/[email protected], which provided a new strategy for the analysis field of glucose.
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