微通道
微流控
水溶液
双水相体系
相(物质)
传质
化学
扩散
流量(数学)
液态液体
电场
纳米技术
色谱法
材料科学
生物系统
化学物理
机械
有机化学
热力学
物理
生物
量子力学
作者
Steffen Hardt,Thomas Hahn
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2012-01-01
卷期号:12 (3): 434-442
被引量:159
摘要
An overview is given about research activities in which aqueous two phase systems (ATPSs) are utilized in microfluidic setups. ATPSs consist of two immiscible aqueous phases and have traditionally been used for the separation and purification of biological material such as proteins or cells. Microfluidic implementations of such schemes are usually based on a number of co-flowing streams of immiscible phases in a microchannel, thereby replacing the standard batch by flow-through processes. Some aspects of the stability of such flow patterns and the recovery of the phases at the channel exit are reviewed. Furthermore, the diffusive mass transfer and sample partitioning between the phases are discussed, and corresponding applications are highlighted. When diffusion is superposed by an applied electric field normal to the liquid/liquid interface, the transport processes are accelerated, and under specific conditions the interface acts as a size-selective filter for molecules. Finally, the activities involving droplet microflows of ATPSs are reviewed. By either forming ATPS droplets in an organic phase or a droplet of one aqueous phase inside the other, a range of applications has been demonstrated, extending from separation/purification schemes to the patterning of surfaces covered with cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI