拟南芥
生物
赤霉素
胚根
发芽
基因
生物合成
串扰
植物激素
赤霉素
基因表达
生物化学
遗传学
植物
突变体
物理
光学
作者
Mikihiro Ogawa,Atsushi Hanada,Yukika Yamauchi,Ayuko Kuwahara,Yuji Kamiya,Shinjiro Yamaguchi
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2003-07-01
卷期号:15 (7): 1591-1604
被引量:867
摘要
Abstract The hormone-mediated control of plant growth and development involves both synthesis and response. Previous studies have shown that gibberellin (GA) plays an essential role in Arabidopsis seed germination. To learn how GA stimulates seed germination, we performed comprehensive analyses of GA biosynthesis and response using gas chromatography–mass spectrometry and oligonucleotide-based DNA microarray analysis. In addition, spatial correlations between GA biosynthesis and response were assessed by in situ hybridization. We identified a number of transcripts, the abundance of which is modulated upon exposure to exogenous GA. A subset of these GA-regulated genes was expressed in accordance with an increase in endogenous active GA levels, which occurs just before radicle emergence. The GA-responsive genes identified include those responsible for synthesis, transport, and signaling of other hormones, suggesting the presence of uncharacterized crosstalk between GA and other hormones. In situ hybridization analysis demonstrated that the expression of GA-responsive genes is not restricted to the predicted site of GA biosynthesis, suggesting that GA itself, or GA signals, is transmitted across different cell types during Arabidopsis seed germination.
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