Dual‐Color Imaging of Sodium/Calcium Ion Activities with Two‐Photon Fluorescent Probes

A two-photon probe (BCaM) shows 14-fold enhancement in two-photon emission fluorescence in response to Ca2+ and shows high sensitivity and selectivity for near-membrane Ca2+ ions (see picture). Combined with the known Na+ two-photon probe ANa1, BCaM allows simultaneous dual-color imaging of Ca2+/Na+ activities within live cells and in tissues at more than 100 μm depth for long time periods without photobleaching. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.