Clinical significance of antinuclear antibodies. Comparison of detection with immunofluorescence and enzyme‐linked immunosorbent assays

抗核抗体 免疫荧光 间接免疫荧光 抗体 医学 临床意义 免疫学 分子生物学 自身抗体 病理 生物
作者
W Emlen,Laurie O’Neill
出处
期刊:Arthritis & Rheumatism [Wiley]
卷期号:40 (9): 1612-1618 被引量:137
标识
DOI:10.1002/art.1780400910
摘要

Abstract Objective . To compare the measurement of antinuclear antibodies (ANA) by immunofluorescence and by 6 different commercial enzyme‐linked immunosorbent assays (ELISAs) in clearly defined patient groups and serum samples. Methods . Serum samples were derived from 3 sources: 1) patients with a known clinical diagnosis of systemic lupus erythematosus (SLE) (group 1), 2) sera with known monospecific ANA reactivity (group 2), and 3) sera from consecutive patients for whom ANA testing had been ordered (group 3). Each of these sera was tested for the presence of ANA by immunofluorescence on HEp‐2 cells, and by using each of 6 commercially available ELISA ANA kits. Results . In patients with known clinical SLE, 88% were ANA positive by immunofluorescence. Positivity with the different ELISAs ranged from 62% to 90%. While most ELISAs successfully detected antibodies to SS‐A, RNP, and Sm, there were significant differences between assays in the detection of anticentromere antibodies and anti‐DNA. Measurement of ANA in consecutive patients for whom an ANA test was requested showed that, generally, those assays with high sensitivity for detection of SLE had a high false‐positive rate, whereas those assays with a low false‐positive rate failed to identify some patients with a clinical diagnosis of SLE. Conclusion . There are significant differences in the detection of ANA by immunofluorescence and different ELISA kits. Agreement between different assays is generally marginal. When ordering and interpreting an ANA test, the clinician must be familiar with the specific assay being used to measure ANA and the differences between the various ANA assays.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
努力搬砖努力干完成签到,获得积分10
刚刚
刚刚
1秒前
夏天发布了新的文献求助20
1秒前
小鸭子发布了新的文献求助10
1秒前
wjm发布了新的文献求助10
2秒前
2秒前
雪寒完成签到,获得积分10
2秒前
Owen应助swordlee采纳,获得10
2秒前
小平头啤酒肚完成签到,获得积分10
3秒前
咿咿呀呀发布了新的文献求助10
3秒前
Lucas应助Nightfall采纳,获得10
3秒前
3秒前
4秒前
高高的汝燕完成签到,获得积分10
4秒前
悦耳凡柔发布了新的文献求助20
5秒前
max发布了新的文献求助10
5秒前
Anxietymaker发布了新的文献求助10
6秒前
完美世界应助王京华采纳,获得10
6秒前
西西完成签到,获得积分10
8秒前
彩虹天堂发布了新的文献求助10
9秒前
10秒前
10秒前
玛卡巴卡发布了新的文献求助10
10秒前
11秒前
咿咿呀呀完成签到,获得积分10
11秒前
Anxietymaker完成签到,获得积分10
12秒前
biov完成签到,获得积分10
12秒前
LYY完成签到,获得积分10
13秒前
13秒前
狂野怜蕾发布了新的文献求助10
13秒前
14秒前
qq.com发布了新的文献求助10
15秒前
15秒前
123发布了新的文献求助10
16秒前
谨慎含双发布了新的文献求助10
16秒前
李健的小迷弟应助gyhk采纳,获得10
18秒前
18秒前
18秒前
MOON完成签到,获得积分10
18秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546508
求助须知:如何正确求助?哪些是违规求助? 3123622
关于积分的说明 9356111
捐赠科研通 2822292
什么是DOI,文献DOI怎么找? 1551314
邀请新用户注册赠送积分活动 723303
科研通“疑难数据库(出版商)”最低求助积分说明 713699