响应面法
中心组合设计
作文(语言)
发酵
工业发酵
腺苷
析因实验
分式析因设计
化学
色谱法
食品科学
生物化学
数学
语言学
统计
哲学
作者
Xiaochun Chen,Jianxin Bai,Jiaming Cao,Zhenjiang Li,Jing Xiong,Lei Zhang,Hong Yuan,Hanjie Ying
标识
DOI:10.1016/j.biortech.2008.07.062
摘要
Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3′,5′-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (211 − 7) was applied to evaluate the effects of different components in the medium. K2HPO4, MgSO4 and NaF were found to significantly influence on the cAMP production. The steepest ascent method was used to access the optimal region of the medium composition. The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology. The optimal medium composition to achieve the optimal cAMP production was determined (g/L): K2HPO4, 12.78; MgSO4, 3.53 and NaF, 0.18. The corresponding cAMP concentration was 8.50 g/L, which was about 1.8-fold increase compared with that using the original medium. Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The cAMP fermentation in 5 L fermenter reached 9.87 g/L.
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