菊花
生物
脯氨酸
互补DNA
基因
耐旱性
植物
非生物胁迫
盐度
基因表达
转基因作物
转基因
cDNA末端的快速扩增
分子生物学
遗传学
氨基酸
分子克隆
生态学
作者
Wenjie Gao,Mingyue He,Jie Liu,Xin Ma,Yu Zhang,Silan Dai,Yunwei Zhou
标识
DOI:10.1016/j.plaphy.2018.01.004
摘要
This paper reports the first study on plant CBF transcription factors (TF) in salt and drought stress responses in Chrysanthemum lavandulifolium. A CBF homolog gene, named as ClCBF1, from C. lavandulifolium was isolated using rapid amplification of cDNA ends (RACE). The deduced peptide is comprised of 210 amino acids (AA) containing an AP2 structural domain characteristic of the AP2 gene family. Quantitative real-time PCR revealed that ClCBF1 gene exhibit differential expression patterns across root, leaf and stem tissues, and it was strongly induced under salt and drought treatments of C. lavandulifolium. Overexpression of ClCBF1 in C. morifolium ‘White Snow’ resulted in stronger tolerance to salt and drought stresses. The ClCBF1 expression level, enzymatic activities of superoxide dismutase and peroxidase, and contents of proline and soluble proteins were enhanced in these transgenic lines, they were repressed in the antisense transgenic lines under the same stress conditions. Results indicate that ClCBF1 represents a promising candidate gene in improving abiotic stress tolerance among ornamental plants.
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