高分子
水溶液
乙二醇
聚合物
相(物质)
化学工程
膜
化学
右旋糖酐
双水相体系
材料科学
色谱法
有机化学
生物化学
工程类
作者
G Johansson,Harry Walter
出处
期刊:International review of cytology
日期:1999-01-01
卷期号:: 33-60
被引量:43
标识
DOI:10.1016/s0074-7696(08)60521-5
摘要
Aqueous phase separation is a general phenomenon which occurs when structurally distinct water-soluble macromolecules are dissolved, above certain concentrations, in water. The number of aqueous phases obtained depends on the number of such distinct macromolecular species used. Aqueous two-phase systems, primarily those containing poly(ethylene glycol) and dextran, have been widely used for the separation of biomaterials (macromolecules, membranes, organelles, cells) by partitioning. The polymer and salt compositions and concentrations chosen greatly affect the physical properties of the phases. These, in turn, interact with the physical properties of biomaterials included in the phases and affect their partitioning. Specific extractions of biomaterials can be effected by including affinity ligands in the systems. The phase systems can also be used to obtain information on the surface properties of materials partitioned in them; to study interactions between biomaterials; and to concentrate such materials.
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