MHC I级
主要组织相容性复合体
肽
MHC限制
β-2微球蛋白
CD8型
三聚体
抗原呈递
重链
化学
免疫球蛋白轻链
抗原
生物
分子生物学
生物化学
免疫学
抗体
细胞毒性T细胞
体外
二聚体
有机化学
基因
作者
Ted H. Hansen,Yanbo Yu,Daved H. Fremont
标识
DOI:10.1002/0471142735.im1705s87
摘要
This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.
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