Abstract The present study aims to prepare and characterize quercetin doped nanoemulsion (QTNE). On the basis of results of solubility, physicochemical interaction (FTIR, RAMAN, DSC-TGA and P-XRD) and morphological (FESEM) studies, CAP MCM NF and CR RH 40 were selected as oil and surfactant. In-vitro drug release studies showed that QTNE1 enhanced the release of QT for prolonged period of time and also the QT release from QTNE1 (58.82%) was more as compared to QT suspension (18.12%). In-vitro antioxidant (standard/modified ABTS and DPPH) and antibacterial assay showed that the antioxidant activity (AOA) and the antibacterial activity (ABA) of QTNE1 was significantly more than pure QT (p