透明质酸酶
透明质酸
化学
剂量
色谱法
显著性差异
体外
粒径
食品科学
酶
生物化学
药理学
医学
内科学
解剖
物理化学
作者
Linggang Liang,Lei Pan,Ye Zhao,Yu Ji,Hangyan Shi
标识
DOI:10.3760/cma.j.issn.1671-0290.2017.01.015
摘要
Objective
To study the process of hyaluronidase enzymolysis under different conditions and to provide the basis for clinical application.
Methods
The experiment of 15 hyaluronic acid products from 10 brands was conducted in vitro with 15 concentrations and 4 dosages of hyaluronidase at 37 ℃ and 24 ℃. The results of experiment were compared with the clinical observations.
Results
The duration of enzymolysis was inversely correlated with hyaluronidase concentration. The duration of enzymolysis process reduced 60% when equal hyaluronidase was used as compared with that when half hyaluronidase was used. The enzymolysis duration could be 24 folds in different products. The enzymolysis time was 2.9 folds at 24 ℃ [24 ℃ was (77.67±35.33) min], compared with that at 37 ℃[37 ℃ was (27.13±11.05) min]. It took much more time of enzymolysis in vivo than in vitro for the same product.
Conclusions
Enzymolysis efficiency is positively correlated with the concentration and dosage of enzyme and inversely associated with the particle size and viscosity.
Key words:
Hyaluronic acid; Hyaluronidase; Temperature; Time of enzymolysis
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