Potentially Critical Roles ofNDUFB5,TIMMDC1,and VDAC3in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis

生物 小桶 基因 计算生物学 微阵列分析技术 遗传学 生物信息学 蛋白质亚单位 基因表达 基因本体论
作者
Kai Kang,Jingtian Li,Ruidong Li,Xiufeng Xu,Jianli Liu,Limin Qin,Tao Huang,Jinhua Wu,Min Jiao,Miaomiao Wei,Hongjie Wang,Tao Wang,Quan Zhang
出处
期刊:DNA and Cell Biology [Mary Ann Liebert]
卷期号:39 (1): 105-117 被引量:10
标识
DOI:10.1089/dna.2019.4859
摘要

Septic cardiomyopathy (SC) is a rare and harmful cardiovascular disease with decreased left ventricular (LV) output and multiple organ failure, which poses a serious threat to human life. Despite the advances in SC, its diagnostic basis and treatment methods are limited, and the specific diagnostic biomarkers and its candidate regulatory targets have not yet been fully established. In this study, the GSE79962 gene expression profile was retrieved, with 20 patients with SC and 11 healthy donors as control. Weighted gene coexpression network analysis (WGCNA) was employed to investigate gene modules that were strongly correlated with clinical phenotypes. Blue module was found to be most significantly related to SC. Moreover, Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the coexpression genes in blue module and showed that it was associated with metabolic pathways, oxidative phosphorylation, and cardiac muscle contraction. Furthermore, a total of 10 hub genes NDUFB5, TIMMDC1, VDAC3, COQ10A, MRPL16 (mitochondrial ribosomal protein L16), C3orf43, TMEM182, DLAT, NDUFA8, and PDHB (pyruvate dehydrogenase E1 beta subunit) in the blue module were identified at transcriptional level and further validated at translational level in myocardium of an lipopolysaccharide-induced septic cardiac dysfunction mouse model. Overall, the results of quantitative real-time polymerase chain reaction were consistent with most of the microarray analysis results. Intriguingly, we observed that the highest change was NDUFB5, TIMMDC1, and VDAC3. These identified and validated genes provided references that would advance the understanding of molecular mechanisms of SC. Taken together, using WGCNA, the hub genes NDUFB5, TIMMDC1, and VDAC3 might serve as potential biomarkers for diagnosis and/or therapeutic targets for precise treatment of SC in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
tian完成签到,获得积分10
1秒前
科目三应助欢呼的念露采纳,获得10
1秒前
情怀应助阿童木采纳,获得10
3秒前
雷大帅完成签到 ,获得积分10
3秒前
4秒前
不筝完成签到,获得积分20
4秒前
5秒前
5秒前
6秒前
听白完成签到 ,获得积分10
7秒前
科研通AI2S应助wonder采纳,获得10
7秒前
漂亮钢铁侠应助yyjw采纳,获得15
8秒前
8秒前
8秒前
8秒前
8秒前
9秒前
小蘑菇应助帅气的绿凝采纳,获得10
11秒前
11秒前
12秒前
小马甲应助受伤芝麻采纳,获得10
12秒前
zhiyuan发布了新的文献求助10
13秒前
自信寒蕾发布了新的文献求助10
13秒前
飞云之下发布了新的文献求助10
13秒前
霸气的凝竹完成签到,获得积分10
13秒前
CHEN发布了新的文献求助10
13秒前
14秒前
冷艳如柏发布了新的文献求助10
14秒前
黄明霞发布了新的文献求助10
15秒前
16秒前
hcl发布了新的文献求助10
16秒前
marco完成签到 ,获得积分10
16秒前
17秒前
单薄碧灵完成签到 ,获得积分10
17秒前
Leyi发布了新的文献求助10
17秒前
科研通AI2S应助li采纳,获得10
17秒前
18秒前
20秒前
隐形曼青应助飞云之下采纳,获得30
20秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3247916
求助须知:如何正确求助?哪些是违规求助? 2891121
关于积分的说明 8266358
捐赠科研通 2559345
什么是DOI,文献DOI怎么找? 1388162
科研通“疑难数据库(出版商)”最低求助积分说明 650698
邀请新用户注册赠送积分活动 627590