Practical Issues in High‐Speed Cell Sorting

Abstract Modern flow cytometric cell sorters are all capable of so‐called “high‐speed sorting.” However, there is confusion about exactly how fast a “high‐speed” cell sorter can sort cells. There are many considerations in achieving the fastest sorting speed, as well as the highest quality sort results—cell recovery, purity, and functionality. This requires the same considerations required for “slow‐speed sorting”; however, a more precise implementation is required for high‐speed sorting. The modern cell sorters enable high‐speed sorting because of advances in high‐speed electronics and data processing. We discuss the practical considerations of high‐speed sorting in terms of the theory and practical aspects of the mechanical and software components of sorting, statistics of sorting, cell preparation and viability, instrument setup, sort strategies, and biosafety. Curr. Protoc. Cytom . 51:1.24.1‐1.24.30. © 2010 by John Wiley & Sons, Inc.