Stimulation by Exosomes from Hypoxia Preconditioned Human Umbilical Vein Endothelial Cells Facilitates Mesenchymal Stem Cells Angiogenic Function for Spinal Cord Repair

间充质干细胞 干细胞 微泡 血管生成 医学 脊髓损伤 细胞生物学 癌症研究 免疫学 生物 病理 脊髓 小RNA 生物化学 基因 精神科
作者
Liming Li,Jiafu Mu,Yu Zhang,Chenyang Zhang,Teng Ma,Lu Chen,Tianchen Huang,Jiahe Wu,Jian Cao,Shiqing Feng,Youzhi Cai,Min Han,Jianqing Gao
出处
期刊:ACS Nano [American Chemical Society]
卷期号:16 (7): 10811-10823 被引量:68
标识
DOI:10.1021/acsnano.2c02898
摘要

Revascularization treatment is a critical measure for tissue engineering therapies like spinal cord repair. As multipotent stem cells, mesenchymal stem cells (MSCs) have proven to regulate the lesion microenvironment through feedback to the microenvironment signals. The angiogenic capacities of MSCs have been reported to be facilitated by vein endothelial cells in the niche. As emerging evidence demonstrated the roles of exosomes in cell–cell and cell–microenvironment communications, to cope with the ischemia complication for treatment of traumatic spinal cord injury, the study extracts the microenvironment factors to stimulate angiogenic MSCs through using exosomes (EX) derived from hypoxic preconditioned (HPC) human umbilical vein endothelial cells (HUVEC). The HPC treatment with a hypoxia time segment of only 15 min efficiently enhanced the function of EX in facilitating MSCs angiogenesis activity. MSCs stimulated by HPC-EX showed significant tube formation within 2 h, and the in vivo transplantation of the stimulated MSCs elicited effective nerve tissue repair after rat spinal cord transection, which could be attributed to the pro-angiogenic and anti-inflammatory impacts of the MSCs. Through the simulation of MSCs using HPC-tailored HUVEC exosomes, the results proposed an efficient angiogenic nerve tissue repair strategy for spinal cord injury treatment and could provide inspiration for therapies based on stem cells and exosomes.
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