色谱法
化学
甲酸
蛋白质沉淀
电喷雾电离
乙腈
质谱法
三级四极质谱仪
人血浆
串联质谱法
液相色谱-质谱法
选择性反应监测
高效液相色谱法
作者
Xiaofei Wu,Fan Zhang,Aijing Liu,Mengyang Yu,Hongyun Wang
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2022-05-01
卷期号:14 (10): 715-724
标识
DOI:10.4155/bio-2022-0014
摘要
Background: SOMCL-15-290 is a novel inhibitor that targets FGF receptor, CSF1 receptor and VEGF receptor (kinase insert domain receptor). Aim: This study was aiming at developing a specific high performance liquid chromatography-MS/MS method for quantifying SOMCL-15-290 in human plasma and supporting the first-in-human study. Methods: Plasma samples were prepared using the protein precipitation method and separated on a C18 110A column with acetonitrile and 0.2% formic acid solution as mobile phases. Quantification of SOMCL-15-290 was operated on an Xevo-TQS triple quadrupole tandem mass spectrometer in electrospray ionization positive mode. Results & conclusion: The validated determination method of SOMCL-15-290 has proved feasible and was successfully utilized in the first-in-human study of SOMCL-15-290 in advanced solid tumor patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI