Acute Tumor Inflammation with CD4/8+ and CD11+ Prolong The Survival Effect Induced by Intratumral Injection Optimum Combination of Chemotherapy Drugs with Hydralazine as Hapten in Animal Model

Purpose: To study the survival time of mice with tumor by intratumoral injection with chemotherapy drug Adr plus hapten of DNP or Hydra and find a combination of drugs of Adr with DNP or Hydra can prolong their life, and which one of DNP or Hydra can provide a long life extension of mice with tumor. Method: After inoculation of tumor cells into mice, tumor reach at 4~5mm size as the experiment day 0, the random groups was performed for intratumoral injection with corresponding drugs combination of Adr+DNP or Hydra and Adr or ARA-C control group, follow up to 90 days to find which combination of drugs could provide a longer their life extension of mice with tumor. Result: Comparison the body weight between the groups, there is not significantly different but the control group maintains the stable of weight. it indicated that PYM could not alone induce a expression of immunity at all and DNP as hapten is playing an important role in stimulation of immunity reaction. There is a significant difference between the tumor volume in treatment group and control group from day1 to day 41, it showed that local injection of combination drug can control tumor growth in the difference drugs group. There is a significant difference between survival time in treatment group and the control group. The life extension rate is 31.4% for group of A1 (Adr+Hydra I) is bigger than A2 (Adr+Hydra II) which has same formulation with high dose of Hydra, and A3 (Adr+Ara-C+Hydra) which has one more drug of Ara-CA2; life extension rate for B group, B1 (Adr+DNP) is 24.4%, it is bigger than B2 (Adr+Ara-C+DNP) which has one more drug of Ara-C; Both of A1 and B1 is better than D (Adr+Ara-C) in the life extension rate, it indicated that best Hydra dose in A1 and DNP in B1 played an important role in the life extension, Hydra is a better hapten than DNP. Conclusion: It has further confirmed the abscopal effect induced by chemotherapy drug Adr plus DNP or Hydra is optimum combination with compatibility and could prolong their life of mice, it offer a novel eclectic approach for treating cancer through the Hydra modify with TAA in vivo with intratumoral injection best combination with chemotherapy drug.