The aim of the study was to determine the effect of osmoconcentration in a sucrose and sodium chloride solution on the efficiency of lactic fermentation and the content of polyphenols and oligosaccharides in yellow and red onion varieties: Alonso, Hysky, Hystore, and Red Lady. In most cases, no negative effect of onion dehydration was noted on the growth or number of the bacteria tested. Osmotic dehydration of onions prior to lactic fermentation may positively modify the profile of lactic acid isomers by increasing the proportion of the L (+) isomer. The use of osmotic dehydration before fermentation did not adversely affect the content of polyphenols in the onions. Simultaneously, the loss of fructo-oligosaccharides was limited: 60 % of the initial fructo-oligosaccharide content was obtained using the Alonso cultivar and Levilactobacillus brevis 0944 for onion fermentation.