Enrichment of antiplatelet peptides and removal of fishy odor from silver carp skin collagen hydrolysates by macroporous resins: pH value of loading sample affects the peptides separation

Enrichment of antiplatelet peptides from silver carp skin collagen hydrolysates (CH) was studied using macroporous resins. Static adsorption showed that XAD-16 resin was the suitable resin due to its high adsorption capacity. The dynamic desorption of CH was studied on XAD-16 resin by ethanol gradient elution. Interestingly, pH value of loading sample had a great impact on the peptides separation. Results revealed that the yield and the antiplatelet activity of Ethl-20% fraction were highest at loading sample pH 6.0. The antiplatelet peptides were enriched in the 20% ethanol fraction with IC50 2.03 mg/mL compared to IC50 of CH, 4.7 mg/mL. Besides, the Ethl-20% fraction had a weakest fishy odor. Moreover, a series of peptides containing Hyp-Gly or Pro-Gly were identified from Ethl-20% fraction, which contributed to the antiplatelet activities. This study provided a simple and efficient method for large-scale separation enrichment of antiplatelet peptides as functional foods from CH.