Blood-liquid extract inhibits bacteria through diffusion-mediated nano-CuO

This study's successful preparation of copper oxide nanoparticles (CuO NPs) involved combining a blood liquid extractor with Cu2NO3 salt via a hydrothermal process. CuO NPs are essential to the antibacterial process because they eradicate germs and provide a disease-free atmosphere. XRD analysis, FE-SEM images, TEM, EDX, Zeta potential, UV–visible spectra, and FT-IR spectra were employed to investigate CuO NPs. According to the XRD data, CuO NPs have an FCC structure and a crystallite size range from 9.3 to 18 nm. Diffraction peaks for CuO NPs may be assigned high crystal quality. Furthermore, the FE-SEM data showed that the CuO NPs had a tiny grain size of between 15 and 33 nm. TEM scans revealed that CuO NPs were spherical in shape and ranged in size from 11.6 to 20 nm. EDX spectra with their image showed CuO purity with Cu and O occurrences. The symmetrical stretching vibrations of Cu-O bonds exhibit peaks at 626 cm−1 in the FT-IR spectra. With respect to zeta potential result was −21.9 mV. The UV–visible spectrum is used to identify the energy band gap of 5.3 eV of CuO NPs. Utilizing the diffusion method, the inhibition zones of antibacterials using CuO NPs were assessed. At high concentrations, the inhibition zones measured 29.5 mm for gram-positive bacteria (Staphylococcus aureus) and 32.5 mm for gram-negative bacteria (Escherichia coli); at lower concentrations, the inhibition zone diameters (IZDs) measured by minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests were 4.2±0.24 mm for gram-positive bacteria (Staphylococcus aureus) and 14±0.13 mm for gram-negative bacteria (Escherichia coli). The mixture of blood liquid extractor with Cu2NO3 salt via a hydrothermal process is quite a new idea, and it has not yet appeared in any research papers to the author's knowledge.