Tetrahydropalmatine promotes random skin flap survival in rats via the PI3K/AKT signaling pathway

PI3K/AKT/mTOR通路 蛋白激酶B 信号转导 延胡索乙素 医学 药理学 传统医学 生物 细胞生物学 病理 替代医学 延胡索 中医药
作者
Jialong Yang,Jiapeng Deng,Kaitao Wang,An Wang,Guodong Chen,Chen Qing-yu,Minle Ye,Xinyu Wu,Xinye Wang,Dingsheng Lin
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:324: 117808-117808 被引量:10
标识
DOI:10.1016/j.jep.2024.117808
摘要

Flap necrosis is the most common complication after flap transplantation, but its prevention remains challenging. Tetrahydropalmatine (THP) is the main bioactive component of the traditional Chinese medicine Corydalis yanhusuo, with effects that include the activation of blood circulation, the promotion of qi, and pain relief. Although THP is widely used to treat various pain conditions, its impact on flap survival is unknown. To explore the effect and mechanism of THP on skin flap survival. In this study, we established a modified McFarlane flap model, and the flap survival rate was calculated after 7 days of THP treatment. Angiogenesis and blood perfusion were evaluated using lead oxide/gelatin angiography and laser Doppler, respectively. Flap tissue obtained from zone II was evaluated histopathologically, by hematoxylin and eosin staining, and in assays for malondialdehyde content and superoxide dismutase activity. Immunofluorescence was performed to detect interleukin (IL)-6, tumor necrosis factor (TNF)-α, hypoxia-inducible factor (HIF)-1α, Bcl-2, Bax, caspase-3, caspase-9, SQSTM1/P62, Beclin-1, and LC3 expression, and Western blot to assess PI3K/AKT signaling pathway activation and Vascular endothelial growth factor (VEGF) expression. The role played by the autophagy pathway in flap necrosis was examined using rapamycin, a specific inhibitor of mTOR. Experimentally, THP improved the survival rate of skin flaps, promoted angiogenesis, and improved blood perfusion. THP administration reduced the inflammatory response, oxidative stress, and apoptosis in addition to inhibiting autophagy via the PI3K/AKT/mTOR pathway. Rapamycin partially reversed these effects. THP promotes skin flap survival via the PI3K/AKT signaling pathway.
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