Shear stress activates the Piezo1 channel to facilitate valvular endothelium-oriented differentiation and maturation of human induced pluripotent stem cells

压电1 材料科学 诱导多能干细胞 细胞生物学 干细胞 剪应力 蛋白激酶B PI3K/AKT/mTOR通路 内皮干细胞 化学 信号转导 内科学 医学 复合材料 生物 胚胎干细胞 体外 离子通道 基因 生物化学 受体 机械敏感通道
作者
Minghui Xie,Hong Cao,Weihua Qiao,Ge Yan,Xingyu Qian,Yecen Zhang,Xu Li,Shuyu Wen,Jiawei Shi,Min Cheng,Nianguo Dong
出处
期刊:Acta Biomaterialia [Elsevier BV]
卷期号:178: 181-195 被引量:2
标识
DOI:10.1016/j.actbio.2024.02.043
摘要

Valvular endothelial cells (VECs) derived from human induced pluripotent stem cells (hiPSCs) provide an unlimited cell source for tissue engineering heart valves (TEHVs); however, they are limited by their low differentiation efficiency and immature function. In our study, we applied unidirectional shear stress to promote hiPSCs differentiation into valvular endothelial-like cells (VELs). Compared to the static group, shear stress efficiently promoted the differentiation and functional maturation of hiPSC-VELs, as demonstrated by the efficiency of endothelial differentiation reaching 98.3% in the high shear stress group (45 dyn/cm2). Furthermore, we found that Piezo1 served as a crucial mechanosensor for the differentiation and maturation of VELs. Mechanistically, the activation of Piezo1 by shear stress resulted in the influx of calcium ions, which in turn initiated the Akt signaling pathway and promoted the differentiation of hiPSCs into mature VELs. Moreover, VELs cultured on decellularized heart valves (DHVs) exhibited a notable propensity for proliferation, robust adhesion properties, and antithrombotic characteristics, which were dependent on the activation of the Piezo1 channel. Overall, our study demonstrated that proper shear stress activated the Piezo1 channel to facilitate the differentiation and maturation of hiPSC-VELs via the Akt pathway, providing a potential cell source for regenerative medicine, drug screening, pathogenesis, and disease modeling. This is the first research that systematically analyzes the effect of shear stress on valvular endothelial-like cells (VELs) derived from human induced pluripotent stem cells (hiPSCs). Mechanistically, unidirectional shear stress activates Piezo1, resulting in an elevation of calcium levels, which triggers the Akt signaling pathway and then facilitates the differentiation of functional maturation VELs. After exposure to shear stress, the VELs exhibited enhanced proliferation, robust adhesion capabilities, and antithrombotic characteristics while being cultured on decellularized heart valves. Thus, it is of interest to develop hiPSCs-VELs using shear stress and the Piezo1 channel provides insights into the functional maturation of valvular endothelial cells, thereby serving as a catalyst for potential applications in the development of therapeutic and tissue-engineered heart valves in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
magictoo发布了新的文献求助30
4秒前
6秒前
yang完成签到,获得积分10
6秒前
Minicoper发布了新的文献求助10
7秒前
快乐丸子完成签到,获得积分10
8秒前
简单而复杂完成签到,获得积分10
8秒前
大橙子发布了新的文献求助10
12秒前
张牧之完成签到 ,获得积分10
14秒前
冷冷暴力完成签到,获得积分10
16秒前
YYY完成签到,获得积分10
16秒前
16秒前
gujian完成签到 ,获得积分10
19秒前
帅气的秘密完成签到 ,获得积分10
20秒前
自然函发布了新的文献求助10
24秒前
冰冰双双完成签到,获得积分10
24秒前
开心夏旋完成签到 ,获得积分0
26秒前
我要读博士完成签到 ,获得积分10
29秒前
活泼的大船完成签到,获得积分10
29秒前
AFF完成签到,获得积分10
30秒前
31秒前
无私小小完成签到,获得积分10
32秒前
随心所欲完成签到 ,获得积分10
33秒前
润润轩轩完成签到 ,获得积分10
34秒前
CodeCraft应助大橙子采纳,获得10
34秒前
ZR完成签到,获得积分10
35秒前
magictoo完成签到,获得积分10
35秒前
陈昊发布了新的文献求助10
36秒前
zhangliangfu完成签到 ,获得积分10
36秒前
金石为开完成签到,获得积分10
36秒前
王QQ完成签到 ,获得积分10
38秒前
唐唐完成签到 ,获得积分10
42秒前
最棒哒完成签到 ,获得积分10
42秒前
鸣鸣完成签到,获得积分10
43秒前
123321完成签到 ,获得积分10
44秒前
卓若之完成签到 ,获得积分10
45秒前
苯二氮卓完成签到,获得积分10
46秒前
温暖完成签到 ,获得积分10
48秒前
mojomars完成签到,获得积分10
48秒前
时尚雨兰完成签到,获得积分0
49秒前
一叶知秋完成签到,获得积分10
51秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038128
求助须知:如何正确求助?哪些是违规求助? 3575831
关于积分的说明 11373827
捐赠科研通 3305610
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022