Association of Short‐Chain Fatty Acids with Gut Microbiota and Lipid Metabolism in Mice with Diarrhea Induced by High‐Fat Diet in a Fatigued State

Scope Preliminary research finds that a high‐fat diet (HFD) in a fatigued state triggers diarrhea, but the exact mechanism has not been clarified. To address concerns about the pathogenesis of diarrhea, the study evaluates the composition and metabolomics of the gut microbiota. Methods and results The study uses the multiple platform apparatus device to induce fatigue in mice, combined with intragastric administration of lard‐caused diarrhea. Subsequently, the characteristics and interaction relationship of gut microbiota, short‐chain fatty acids (SCFAs), inflammatory biomarkers, brain‐gut peptides, and lipid metabolism are analyzed at the end of the experiment. HFD in a fatigued state results in a significant increase in interleukin‐17, interleukin‐6, cholecystokinin, somatostatin, and malondialdehyde content in mice ( p < 0.05), along with a substantial decrease in high‐density lipoprotein ( p < 0.05). Additionally, an HFD in a fatigued state causes changes in the structure and composition of the gut microbiota, with Lactobacillus murinus as its characteristic bacteria, and reduces the production of SCFAs. Conclusions An HFD in a fatigued state triggers diarrhea, possibly associated with gut content microbiota dysbiosis, SCFAs deprivation, increased inflammation, and dysregulated lipid metabolism.