化学
乙腈
相(物质)
替考拉宁
传质
甲醇
氨基酸
手性固定相
多孔性
色谱法
化学工程
有机化学
细菌
工程类
生物
万古霉素
金黄色葡萄球菌
生物化学
遗传学
作者
Simona Felletti,Chiara De Luca,Giulia Mazzoccanti,Omar H. Ismail,Simone Manetto,Flavio A. Franchina,Tatiana Chenet,Luisa Pasti,Alberto Cavazzini,Martina Catani
标识
DOI:10.1021/acs.analchem.3c01344
摘要
The retention behavior of small molecules and N-protected amino acids on a zwitterionic teicoplanin chiral stationary phase (CSP), prepared on superficially porous particles (SPPs) of 2.0 μm particle diameter, has shown that efficiency and enantioselectivity, and so enantioresolution, dramatically change depending on the employed organic modifier. In particular, it was found that while methanol permits the boost of enantioselectivity and resolution of the amino acids, at the cost of efficiency, acetonitrile allows for the ability to reach extraordinary efficiency even at high flow rates (with reduced plate height <2 and up to 300,000 plates/m at the optimum flow rate). To understand these features, an approach based on the investigation of mass transfer through the CSP, the estimation of the binding constants of amino acids on the CSP, and the assessment of compositional properties of the interfacial region between bulk mobile phase and solid surface has been adopted.
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