清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

SlPHL1 is involved in low phosphate stress promoting anthocyanin biosynthesis by directly upregulation of genes SlF3H, SlF3′H, and SlLDOX in tomato

拟南芥 生物 生物化学 电泳迁移率测定 基因沉默 转录因子 花青素 发起人 基因 抑制因子 生物合成 基因表达 黄烷酮 突变体 结构基因 分子生物学 细胞生物学 植物 类黄酮 抗氧化剂
作者
Xue‐Qian Wu,Zhongjuan Liu,Yanan Liu,Enhui Wang,Duanmei Zhang,Shaoxuan Huang,Chengquan Li,Yijing Zhang,Zhongze Chen,Yongqiang Zhang
出处
期刊:Plant Physiology and Biochemistry [Elsevier BV]
卷期号:200: 107801-107801 被引量:6
标识
DOI:10.1016/j.plaphy.2023.107801
摘要

Phosphate (Pi) deficiency is a common stress that limits plant growth and development. Plants exhibit a variety of Pi starvation responses (PSRs), including anthocyanin accumulation. The transcription factors of the PHOSPHATE STARVATION RESPONSE (PHR) family, such as AtPHR1 in Arabidopsis, play central roles in the regulation of Pi starvation signaling. Solanum lycopersicum PHR1-like 1 (SlPHL1) is a recently identified PHR involved in PSR regulation in tomato, but the detailed mechanism of its participation in Pi starvation-inducing anthocyanin accumulation remains unclear. Here we found that overexpression of SlPHL1 in tomato increases the expression of genes associated with anthocyanin biosynthesis, thereby promoting anthocyanin biosynthesis, but silencing SlPHL1 with Virus Induced Gene Silencing (VIGS) attenuated low phosphate (LP) stress-induced anthocyanin accumulation and expression of the biosynthesis-related genes. Notably, SlPHL1 is able to bind the promoters of genes Flavanone 3-Hydroxylase (SlF3H), Flavanone 3′-Hydroxylase (SlF3′H), and Leucoanthocyanidin Dioxygenase (SlLDOX) by yeast one-hybrid (Y1H) analysis. Furthermore, Electrophoretic Mobility Shift Assay (EMSA) and transient transcript expression assay showed that PHR1 binding t (sequence (P1BS) motifs located on the promoters of these three genes are critical for SlPHL1 binding and enhancing the gene transcription. Additionally, allogenic overexpression of SlPHL1 could promote anthocyanin biosynthesis in Arabidopsis under LP conditions through the similar mechanism to AtPHR1, suggesting that SlPHL1 might be functionally conserved with AtPHR1 in this process. Taken together, SlPHL1 positively regulates LP-induced anthocyanin accumulation by directly promoting the transcription of SlF3H, SlF3′H and SlLDOX. These findings will contribute to understanding the molecular mechanism of PSR in tomato.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
隐形曼青应助科研通管家采纳,获得10
6秒前
ceeray23发布了新的文献求助20
16秒前
orixero应助ceeray23采纳,获得20
51秒前
1233发布了新的文献求助10
54秒前
ChatGPT发布了新的文献求助10
55秒前
JG完成签到 ,获得积分10
56秒前
jkaaa完成签到,获得积分10
56秒前
1233完成签到,获得积分10
1分钟前
太清完成签到 ,获得积分10
1分钟前
1分钟前
ceeray23发布了新的文献求助20
1分钟前
cadcae完成签到,获得积分10
1分钟前
Eric800824完成签到 ,获得积分10
1分钟前
庚朝年完成签到 ,获得积分10
1分钟前
violetlishu完成签到 ,获得积分10
2分钟前
ceeray23发布了新的文献求助20
2分钟前
yingzaifeixiang完成签到 ,获得积分10
2分钟前
wodetaiyangLLL完成签到 ,获得积分10
2分钟前
称心如意完成签到 ,获得积分10
2分钟前
h41692011完成签到 ,获得积分10
3分钟前
dingding发布了新的文献求助10
3分钟前
今后应助dingding采纳,获得10
4分钟前
Jj7完成签到,获得积分10
4分钟前
4分钟前
4分钟前
4分钟前
4分钟前
彦嘉发布了新的文献求助10
4分钟前
斯文败类应助ceeray23采纳,获得20
5分钟前
打打应助Dave采纳,获得10
5分钟前
5分钟前
5分钟前
ceeray23发布了新的文献求助20
5分钟前
sh1ro发布了新的文献求助10
5分钟前
nojego完成签到,获得积分10
5分钟前
脑洞疼应助科研通管家采纳,获得10
6分钟前
顾矜应助科研通管家采纳,获得10
6分钟前
wjx完成签到 ,获得积分10
6分钟前
John完成签到,获得积分10
6分钟前
顾矜应助ceeray23采纳,获得20
6分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990550
求助须知:如何正确求助?哪些是违规求助? 3532220
关于积分的说明 11256532
捐赠科研通 3271057
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234