Analysis of differentially expressed proteins after EHP-infection and characterization of caspase 3 protein in the whiteleg shrimp (Litopenaeus vannamei)

生物 立陶宛 小虾 肝胰腺 对虾科 养虾业 动物 渔业 生态学 十足目 甲壳动物 水产养殖
作者
Hongtao Liu,Minghui Shen,Yugui He,Bingshun Li,Liang Pu,Guangyuan Xia,Mingqiu Yang,Guofu Wang
出处
期刊:Fish & Shellfish Immunology [Elsevier BV]
卷期号:135: 108698-108698 被引量:4
标识
DOI:10.1016/j.fsi.2023.108698
摘要

Whiteleg shrimp (Litopenaeus vannamei) is the most important species of shrimp farmed worldwide in terms of its economic value. Enterocytozoon hepatopenaei (EHP) infects the hepatopancreas, resulting in the hepatopancreatic microsporidiosis (HPM) of the host, which causes slow growth of the shrimp and poses a threat to the farming industry. In this study, differentially expressed proteins (DEPs) between EHP-infected and uninfected shrimp were investigated through proteomics sequencing. A total of 9908 peptides and 2092 proteins were identified. A total of 69 DEPs were identified in the hepatopancreas (HP), of which, 28 were upregulated and 41 were downregulated. Our results showed that the differences among the level of multiple proteins involved in the apoptosis were significant after the EHP infection, which indicated that the apoptosis pathway was activated in whiteleg shrimp. In addition, expression leve of caspase 3 gene were identified related to the EHP infection. Furthermore, predictions of spatial structure, analysis of phylogeny and chromosome-level linearity of the caspase 3 protein were performed as well. In conclusion, a relatively complete proteomic data set of hepatopancreas tissues in whiteleg shrimp were established in this study. Findings about genes involved in the apoptosis here will provide a further understanding of the molecular mechanism of EHP infection in the internal immunity of whiteleg shrimp.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
NexusExplorer应助古月采纳,获得10
刚刚
星辰大海应助33采纳,获得10
2秒前
彭彭发布了新的文献求助10
4秒前
6秒前
老大蒂亚戈应助潇湘雪月采纳,获得10
7秒前
7秒前
9秒前
9秒前
kk完成签到,获得积分10
9秒前
10秒前
HOPE发布了新的文献求助10
10秒前
AAA完成签到,获得积分10
12秒前
Singularity应助讨厌科研采纳,获得10
12秒前
古月发布了新的文献求助10
13秒前
14秒前
33发布了新的文献求助10
14秒前
汉堡包应助于平川春野采纳,获得10
14秒前
星星发布了新的文献求助10
16秒前
17秒前
赘婿应助十九岁的时差采纳,获得10
17秒前
怠惰vs勤劳完成签到,获得积分10
18秒前
夏天应助青山采纳,获得100
19秒前
20秒前
乖猫要努力应助潇湘雪月采纳,获得10
21秒前
wdy111举报风之星求助涉嫌违规
21秒前
古月完成签到,获得积分10
22秒前
22秒前
23秒前
吴所谓发布了新的文献求助50
26秒前
英吉利25发布了新的文献求助10
27秒前
28秒前
酷波er应助南冥采纳,获得10
28秒前
李沐唅完成签到 ,获得积分10
32秒前
lv完成签到,获得积分10
32秒前
周婷完成签到 ,获得积分10
33秒前
shy完成签到,获得积分10
33秒前
35秒前
犹豫的忆枫完成签到,获得积分10
35秒前
36秒前
夕沫发布了新的文献求助50
36秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989390
求助须知:如何正确求助?哪些是违规求助? 3531487
关于积分的说明 11254109
捐赠科研通 3270153
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809174