Integrated proteomic and phosphoproteomic analysis for characterization of colorectal cancer

小桶 磷酸蛋白质组学 蛋白质组学 生物 计算生物学 结直肠癌 癌变 蛋白质组 定量蛋白质组学 癌症 生物信息学 基因 细胞生物学 基因本体论 生物化学 基因表达 磷酸化 遗传学 蛋白质磷酸化 蛋白激酶A
作者
Huifang Zhu,Yongzhen Li,Jingyu Guo,Feng Shuang,Hong Ge,Chuan-Sha Gu,Sheng Wang,Ruicong Nie,Na Li,Yong‐Xia Wang,Haijun Wang,Jiateng Zhong,Xinlai Qian,Guoyang He
出处
期刊:Journal of Proteomics [Elsevier]
卷期号:274: 104808-104808 被引量:4
标识
DOI:10.1016/j.jprot.2022.104808
摘要

Proteins and translationally modified proteins like phosphoproteins have essential regulatory roles in tumorigenesis. This study attempts to elucidate the dysregulated proteins driving colorectal cancer (CRC). To explore the differential proteins, we performed iTRAQ labeling proteomics and TMT labeling phosphoproteomics analysis of CRC tissues and adjacent non-cancerous tissues. The functions of quantified proteins were analyzed using Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), and Subcellular localization analysis. Depending on the results, we identified 330 differential proteins and 82 phosphoproteins in CRC. GO and KEGG analyses demonstrated that protein changes were primarily associated with regulating biological and metabolic processes through binding to other molecules. Co-expression relationships between proteomic and phosphoproteomic analysis revealed that TMC5, SMC4, SLBP, VSIG2, and NDRG2 were significantly dysregulated differential proteins. Additionally, based on the predicted co-expression proteins, we identified that the stem-loop binding protein (SLBP) was up-regulated in CRC cells and promoted the proliferation and migration of CRC. This study reports an integrated proteomic and phosphoproteomic analysis of CRC to discern the functional impact of protein alterations and provides a candidate diagnostic biomarker or therapeutic target for CRC. Combining one or more high-throughput omics technologies with bioinformatics to analyze biological samples and explore the links between biomolecules and their functions can provide more comprehensive and multi-level insights for disease mechanism research. Proteomics, phosphoproteomics, metabolomics and their combined analysis play an important role in the auxiliary diagnosis, the discovery of biomarkers and novel therapeutic targets for colorectal cancer. In this integrated proteomic and phosphoproteomic analysis, we identified proteins and phosphoproteins in colorectal cancer tissue and analyzed potential mechanisms contributing to progression in colorectal cancer. The results of this study provide a foundation to focus future experiments on the contribution of altered protein and phosphorylation patterns to prevention and treatment of colorectal cancer.
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