Identification of ADPKD-Related Key miRNAs by Constructing a miRNA-mRNA Interaction Network and Experimental Verification

Asthma, DNA methylation inhibitor, 5-Azacytidine, forkhead box P3, T-regulatory cells, epigenetic regulationIt has recently been demonstrated that DNA methylation causes differential secretion of T helper 1 (Th1) and Th2 cytokines by effector T cell polarisation.This study investigates changes in the marker genes of T cell subsets after allergen sensitisation and demethylation intervention as well as the T cells' effects on allergic airway inflammation development.The number of T cell subsets and the expression of cytokines were detected by a well-established model of experimental asthma.The number of T cell subsets was detected by flow cytometry, the protein expression was detected by enzyme-linked immunosorbent assay, and the gene expression was detected by real-time polymerase chain reaction.In addition, in vitro experiments with Jurkat cells were used to verify the effect of methylase inhibitor 5-Aza-2'-deoxycytidine on the expression of cytokines and genes in the T cells.The expression of Th2 cells, cytokine proteins and genes after allergen sensitisation revealed a significant increase in CD4 + T cells.However, analysis of regulatory T cells revealed the opposite results.The decrease of Th2 cells, cytokine proteins, and genes was reversed by a DNA methyltransferase inhibitor in vitro and in vivo, which also increased the allergen sensitisation status and decreased the inflammatory response.Epigenetics has an impact on disease occurrence.Epigenetic regulation in T cells contributes to the development of experimental asthma, which can be treated pharmacologically.