肌成纤维细胞
氯沙坦
纤维化
医学
眼科
基底膜
间质细胞
病理
内科学
血管紧张素II
受体
作者
Lycia Pedral Sampaio,Guilherme Simões Luz Hilgert,Thomas Michael Shiju,Marcony R. Santhiago,Steven E. Wilson
出处
期刊:Journal of Refractive Surgery
[SLACK, Inc.]
日期:2022-12-01
卷期号:38 (12): 820-829
被引量:15
标识
DOI:10.3928/1081597x-20221026-03
摘要
To study the effect of topical losartan compared to vehicle on the generation of myofibroblasts and development of late haze scarring fibrosis after photorefractive keratectomy (PRK) in rabbits.Twelve rabbits had -9.00 diopter (D) PRK in one eye followed by 50 µL of topical 0.2 mg/mL losartan or 50 µL of vehicle six times per day for 1 month. Standardized slit-lamp photographs were obtained prior to death. Duplex immunohistochemistry was performed on cryofixed corneas for myofibroblast marker alpha-smooth muscle actin (α-SMA) and keratocyte marker keratocan or collagen type IV and transforming growth factor (TGF)-β1. ImageJ software (National Institutes of Health) was used for quantitation.Topical losartan compared to vehicle significantly decreased corneal opacity (P = .04) and anterior stromal myofibroblast generation (P = .01) at 1 month after PRK. Topical losartan compared to vehicle also decreased anterior stromal non-basement membrane collagen type IV at 1 month after PRK (P = .004).Topical angiotensin converting enzyme II receptor inhibitor losartan, a known inhibitor of TGF-β signaling, decreased late haze scarring fibrosis and myofibroblast generation after -9.00 D PRK in rabbits compared to vehicle. It also decreases TGF-β-modulated, corneal fibroblast-produced, non-basement membrane stromal collagen type IV-likely also through inhibition of TGF-β signaling. [J Refract Surg. 2022;38(12):820-829.].
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