溶藻弧菌
生物
生物膜
群集运动
微生物学
调节器
运动性
蜂群(蜜蜂)
群体感应
弧菌
细菌
细胞生物学
遗传学
基因
作者
Tongxian Chen,Xiaoling Zhou,Ruonan Feng,Shuhao Shi,Xiyu Chen,Bingqi Wei,Zhong Hu,Tao Peng
出处
期刊:BMC Biology
[BioMed Central]
日期:2024-11-06
卷期号:22 (1)
被引量:2
标识
DOI:10.1186/s12915-024-02057-y
摘要
NorR, as a single-target regulator, has been demonstrated to be involved in NO detoxification in bacteria under anaerobic conditions. Here, the norR gene was identified and deleted in the genome of Vibrio alginolyticus. The results showed that deletion of norR in Vibrio alginolyticus led to lower swarming motility and more biofilm formation on aerobic condition. Moreover, we proved that NorR from E. coli had a similar function in controlling motility. NorR overexpression led to increased resistance to oxidative stress and tetracycline. We also observed a reduced ability of the NorR-overexpressing strain to adapt to iron limitation condition. Transcriptome analysis showed that the genes responsible for bacterial motility and biofilm formation were affected by NorR. The expressions of several sigma factors (RpoS, RpoN, and RpoH) and response regulators (LuxR and MarR) were also controlled by NorR. Furthermore, Chip-qPCR showed that there is a direct binding between NorR and the promoter of rpoS. Based on these results, NorR appears to be a central regulator involved in biofilm formation and swarming motility in Vibrio alginolyticus.
科研通智能强力驱动
Strongly Powered by AbleSci AI