哈卡特
姜黄素
Zeta电位
细胞毒性
超声
体外
化学
粒径
药理学
均质化(气候)
一氧化氮
材料科学
纳米颗粒
纳米技术
色谱法
生物化学
医学
有机化学
物理化学
生物多样性
生物
生态学
作者
Ankit Awasthi,Bimlesh Kumar,Monica Gulati,Sukriti Vishwas,Leander Corrie,Jaskiran Kaur,Rubiya Khursheed,Rozhan Arif Muhammed,Duran Kala,Omji Porwal,Molakpogu Ravindra Babu,MVNL Chaitanya,Ankit Kumar,Narendra Kumar Pandey,Harish Dureja,Dinesh Kumar Chellappan,Niraj Kumar Jha,Gaurav Gupta,Parteek Prasher,Deepak Kumar,Kamal Dua,Sachin Kumar Singh
标识
DOI:10.1007/s11095-022-03401-z
摘要
PurposeThe aim of current study is to formulate, optimize and characterize the developed formulation of Mesalamine-Curcumin Nanostructured Lipid Carriers (Mes-Cur NLCs).MethodsIt was formulated using high pressure homogenization followed by probe sonication and formulation variables were optimized using Central Composite Design. The particle size (PS), zeta potential (ZP), entrapment efficiency (EE), drug release, cytotoxicity on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells and efficacy on RAW264.7 cells for optimized formulation was determined.ResultsThe PS, ZP and EE were found to be 85.26 nm, −23.7 ± 7.45 mV, 99.2 ± 2.62 % (Mes) and 84 ± 1.51 % (Cur), respectively. The good correlation between predicted and obtained value indicated suitability and reproducibility of experimental design. NLCs showed spherical shape as confirmed by TEM. In vitro drug release profile of prepared formulation showed that Mes exhibited 100 % release at 48 h, whereas Cur exhibited 82.23 ± 2.97% release at 120 h. Both the drugs exhibited sustained release upon incorporation into the NLCs. The absence of any significant cell death during MTT assay performed on NIH 3T3 fibroblasts cells and HaCaT keratinocytes cells indicated that NLCs’ were safe for use. Furthermore, significant reduction in nitric oxide level during anti-inflammatory evaluation of formulation on RAW264.7 cells showed excellent potential for the formulation to treat inflammation. The formulation was found stable as no significant difference between the PS, ZP and EE of the fresh and aged NLCs was observed.ConclusionThe outcomes of study deciphered successful formulation of Mes-Cur NLCs.
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