Maintenance of cell viability in extraoral conditions for a case of intentional replantation to retrieve a separated endodontic instrument

Intentional replantation has been used as an alternative treatment modality to tooth extraction and prosthetic replacement when conventional endodontic treatment modalities are unfeasible or contraindicated. This case report presents a successful case of intentional replantation for the mandibular first molar with an endodontic mishap. An endodontic instrument was separated in the apical third of the root canal and extended beyond its mesiobuccal root apex. Intentional replantation served as a means to remove the separated instrument. The periodontal ligament (PDL) cells were kept viable throughout the endodontic treatment using a distinctive technique, wherein a preoperative impression is used for continuous wetting with saline. Platelet-rich fibrin (PRF) was shredded and placed in the alveolar socket to enhance PDL cell reattachment and prevent ankylosis. The 2-year successful follow-up reinforced that intentional replantation can be a viable option for removal of separated instruments that lie beyond the root apex. The use of these techniques to keep PDL cells viable and the use of PRF can aid in prevention of ankylosis.