Synthesis, crystal structure and biological evaluation of new phosphoramide derivatives as urease inhibitors using docking, QSAR and kinetic studies

化学 乙酰羟肟酸 尿素酶 数量结构-活动关系 硫脲 立体化学 对接(动物) 组合化学 有机化学 医学 护理部
作者
Khodayar Gholivand,Mahsa Pooyan,Fahimeh Mohammadpanah,Foroogh Pirastefar,‬Peter C. Junk,Jun Wang,Ali Asghar Ebrahimi Valmoozi,Ahmad Mani‐Varnosfaderani
出处
期刊:Bioorganic Chemistry [Elsevier]
卷期号:86: 482-493 被引量:22
标识
DOI:10.1016/j.bioorg.2019.01.064
摘要

In an attempt to achieve a new class of phosphoramide inhibitors with high potency and resistance to the hydrolysis process against urease enzyme, we synthesized a series of bisphosphoramide derivatives (01-43) and characterized them by various spectroscopic techniques. The crystal structures of compounds 22 and 26 were investigated using X-ray crystallography. The inhibitory activities of the compounds were evaluated against the jack bean urease and were compared to monophosphoramide derivatives and other known standard inhibitors. The compounds containing aromatic amines and their substituted derivatives exhibited very high inhibitory activity in the range of IC50 = 3.4-1.91 × 10-10 nM compared with monophosphoramides, thiourea, and acetohydroxamic acid. It was also found that derivatives with PO functional groups have higher anti-urease activity than those with PS functional groups. Kinetics and docking studies were carried out to explore the binding mechanism that showed these compounds follow a mixed-type mechanism and, due to their extended structures, can cover the entire binding pocket of the enzyme, reducing the formation of the enzyme-substrate complex. The quantitative structure-activity relationship (QSAR) analysis also revealed that the interaction between the enzyme and inhibitor is significantly influenced by aromatic rings and PO functional groups. Collectively, the data obtained from experimental and theoretical studies indicated that these compounds can be developed as appropriate candidates for urease inhibitors in this field.
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