ABO血型系统
注意事项
血型
再现性
医学
全血
抗原
抗体
微量滴定板
离心
计算机科学
色谱法
生物医学工程
化学
免疫学
病理
作者
Hong Zhang,Xiaopei Qiu,Zou Yu-rui,Yanyao Ye,Chao Qi,Lingyun Zou,Yang Xiang,Ke Yang,Yuanfeng Zhu,Yongjun Yang,Yang Zhou,Yang Luo
出处
期刊:Science Translational Medicine
[American Association for the Advancement of Science (AAAS)]
日期:2017-03-15
卷期号:9 (381)
被引量:49
标识
DOI:10.1126/scitranslmed.aaf9209
摘要
Fast and simultaneous forward and reverse blood grouping has long remained elusive. Forward blood grouping detects antigens on red blood cells, whereas reverse grouping identifies specific antibodies present in plasma. We developed a paper-based assay using immobilized antibodies and bromocresol green dye for rapid and reliable blood grouping, where dye-assisted color changes corresponding to distinct blood components provide a visual readout. ABO antigens and five major Rhesus antigens could be detected within 30 s, and simultaneous forward and reverse ABO blood grouping using small volumes (100 μl) of whole blood was achieved within 2 min through on-chip plasma separation without centrifugation. A machine-learning method was developed to classify the spectral plots corresponding to dye-based color changes, which enabled reproducible automatic grouping. Using optimized operating parameters, the dye-assisted paper assay exhibited comparable accuracy and reproducibility to the classical gel-card assays in grouping 3550 human blood samples. When translated to the assembly line and low-cost manufacturing, the proposed approach may be developed into a cost-effective and robust universal blood-grouping platform.
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