Pharmacokinetic studies of chinfloxacin in mouse

Objective To determine the pharmacokinetics of chinfloxacin in mouse,and analyze the PK /PD parameters.Methods Chinfloxacin in mouse plasma after intragastric administration in 5 mg.kg-1 was determined by HPLC-MS / MS with moxifloxacin as internal standard.Methanol-water was used as the mobile phase.Then the parameters were calculated with WinNonlin.Results The Cmax was 1.085 μg.mL-1,Tmax was 10 min,T1 /2 was 1.425 h,AUC0 ～ 24 was 1.101 μg.mL-1.h in mouse plasma,and the Cmax/ MIC50 of S.aureus,S.pneumoniae,H.influenza,H.parainfluenzae and S.pyogenes were all above 8.Conclusion Chinfloxacin was absorbed,distributed and eliminated fast in mouse,and showed good activity against some kinds of bacteria.